Electrochemical screening of the indole/quinolone derivatives as potential protein kinase CK2 inhibitors
New publication: Electrochemical screening of the indole/quinolone derivatives as potential protein kinase CK2 inhibitors
OTAVAchemicals has published new scientific paper on CK2 inhibitors.
Abstract: An electrochemical method based on the bioorganometallic Fc-ATP cosubstrate for kinase-catalyzed phosphorylation reactions was used for monitoring casein kinase 2 (CK2) phosphorylations in the absence and presence of ﬁve indole/quinolone-based potential inhibitors.
Fc-phosphorylation of immobilized peptide RRRDDDSDDD on Au surfaces resulted in a current density at approximately 460 ± 10 mV. An electrochemical redox signal was signiﬁcantly decreased in the presence of inhibitors. In addition, the electrochemical signal was concentration dependent with respect to the potential inhibitors 1 to 5, which proved to be viable CK2 drug targets with estimated IC50 values in the nanomolar range.
PubMed PMID: 22178909